Literature summary for 2.8.2.38 extracted from

Liu, F.; Yang, H.; Wang, L.; Yu, B.
Biosynthesis of the high-value plant secondary product benzyl isothiocyanate via functional expression of multiple heterologous enzymes in Escherichia coli (2016), ACS Synth. Biol., 5, 1557-1565 .

Search for more literature for 2.8.2.38

Application

Application	Comment	Organism
synthesis	3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae are expressed in Escherichia coli and successfully used for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
co-expression of the enzyme with myrosinase from Brevicoryne brassicae in Escherichia coli strain BL21(DE3), expression of codon-optimized sulfotransferase gene SOT18 from Arabidopsis thaliana is successful and can substitute for the insoluble SOT16, desulfoglucosinolate:PAPS sulfotransferase	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and functionally expressed in Escherichia coli. The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli strain BL21(DE3). Reconstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT18, and MYR, respectively, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9C9C9	-	-
Arabidopsis thaliana Col-0	Q9C9C9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies	Arabidopsis thaliana	?	-	-
additional information	usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies	Arabidopsis thaliana Col-0	?	-	-

Synonyms

Synonyms	Comment	Organism
3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase	UniProt	Arabidopsis thaliana
cytosolic sulfotransferase 18	-	Arabidopsis thaliana
SOT18	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)